LOCUS       40924_5564        6495 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Accepter vector pBac3xP3DsRed2attP DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Yonemura N, Tamura T, Uchino K, Kobayashi I, Tatematsu K, Iizuka T, 
            Sezutsu H, Muthulakshmi M, Nagaraju J, Kusakabe T.
  TITLE     PhiC31 integrase-mediated cassette exchange in silkworm embryos
  JOURNAL   Mol. Genet. Genomics 287 (9), 731-739 (2012)
  PUBMED    22842670
REFERENCE   2  (bases 1 to 6495)
  AUTHORS   Yonemura N, Kobayashi I, Uchino K, Sezutsu H, Tamura T, Kusakabe T.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-APR-2012) Contact:Naoyuki Yonemura National Institute 
            of Agrobiological Sciences (NIAS), MAFF; Ohwashi 1-2, Tsukuba 
            Science City 305-8634, Japan
REFERENCE   3  (bases 1 to 6495)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6495)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Genet.
            Genomics"; date: "2012"; volume: "287"; issue: "9"; pages: "731-739"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-APR-2012) Contact:Naoyuki Yonemura National Institute of 
            Agrobiological Sciences (NIAS), MAFF; Ohwashi 1-2, Tsukuba Science 
            City 305-8634, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    705..1473
                     /label=piggyBac right arm
                     /note="piggyBac right arm"
     misc_recomb     complement(1490..1528)
                     /label=Streptomyces phage phiC31 attP sequence
                     /note="Streptomyces phage phiC31 attP sequence"
     protein_bind    1535..1559
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1604..1879
                     /gene="3xP3"
                     /label=Artificial Pax6 responsive promoter
                     /note="Artificial Pax6 responsive promoter"
                     /regulatory_class="promoter"
     gene            1604..1879
                     /gene="3xP3"
                     /label=3xP3
     CDS             1880..2554
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     polyA_signal    2678..2812
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2818..2842)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_recomb     complement(2849..2887)
                     /label=Streptomyces phage phiC31 attP sequence
                     /note="Streptomyces phage phiC31 attP sequence"
     misc_feature    3018..3696
                     /label=piggyBac left arm
                     /note="piggyBac left arm"
     primer_bind     complement(3881..3897)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3905..3921)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3929..3959)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3974..3995)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4283..4871)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5045..5902)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5903..6007)
                     /label=AmpR promoter
ORIGIN
        1 gtgccaagct ttgtttaaaa tataacaaaa ttgtgatccc acaaaatgaa gtggggcaaa
       61 atcaaataat taatagtgtc cgtaaacttg ttggtcttca actttttgag gaacacgttg
      121 gacggcaaat ccgtgactat aacacaagtt gatttaataa ttttagccaa cacgtcgggc
      181 tgcgtgtttt ttgccgacgc gtctgtgtac acgttgatta actggtcgat taaactgttg
      241 aaataattta atttttggtt cttctttaaa tctgtgatga aattttttaa aataacttta
      301 aattcttcat tggtaaaaaa tgccacgttt tgcaacttgt gagggtctaa tatgaggtca
      361 aactcagtag gagttttatc caaaaaagaa aacatgatta cgtctgtaca cgaacgcgta
      421 ttaacgcaga gtgcaaagta taagagggtt aaaaaatata ttttacgcac catatacgca
      481 tcgggttgat atcgttaata tggatcaatt tgaacagttg attaacgtgt ctctgctcaa
      541 gtctttgatc aaaacgcaaa tcgacgaaaa tgtgtcggac aatatcaagt cgatgagcga
      601 aaaactaaaa aggctagaat acgacaatct cacagacagc gttgagatat acggtattca
      661 cgacagcagg ctgaataata aaaaaattag aaactattat ttaaccctag aaagataatc
      721 atattgtgac gtacgttaaa gataatcatg cgtaaaattg acgcatgtgt tttatcggtc
      781 tgtatatcga ggtttattta ttaatttgaa tagatattaa gttttattat atttacactt
      841 acatactaat aataaattca acaaacaatt tatttatgtt tatttattta ttaaaaaaaa
      901 acaaaaactc aaaatttctt ctataaagta acaaaacttt taaacattct ctcttttaca
      961 aaaataaact tattttgtac tttaaaaaca gtcatgttgt attataaaat aagtaattag
     1021 cttaacttat acataataga aacaaattat acttattagt cagtcagaaa caactttggc
     1081 acatatcaat attatgctct cgacaaataa cttttttgca ttttttgcac gatgcatttg
     1141 cctttcgcct tattttagag gggcagtaag tacagtaagt acgttttttc attactggct
     1201 cttcagtact gtcatctgat gtaccaggca cttcatttgg caaaatatta gagatattat
     1261 cgcgcaaata tctcttcaaa gtaggagctt ctaaacgctt acgcataaac gatgacgtca
     1321 ggctcatgta aaggtttctc ataaattttt tgcgactttg aaccttttct cccttgctac
     1381 tgacattatg gctgtatata ataaaagaat ttatgcaggc aatgtttatc attccgtaca
     1441 ataatgccat aggccaccta ttcgtcttcc tacagcttga tggggaattc cccaactggg
     1501 gtaacctttg agttctctca gttgggggtc gatcacaagt ttgtacaaaa aagcaggctt
     1561 cgaaggagat agaaccgggg aaccaattcg ttcccacaat ggttaattcg agctcgcccg
     1621 gggatctaat tcaattagag actaattcaa ttagagctaa ttcaattagg atccaagctt
     1681 atcgatttcg aaccctcgac cgccggagta taaatagagg cgcttcgtct acggagcgac
     1741 aattcaattc aaacaagcaa agtgaacacg tcgctaagcg aaagctaagc aaataaacaa
     1801 gcgcagctga acaagctaaa caatcggggt accgctagag tcgacggtac cgcgggcccg
     1861 ggatccaccg gtcgccacca tggcctcctc cgagaacgtc atcaccgagt tcatgcgctt
     1921 caaggtgcgc atggagggca ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     1981 gggccgcccc tacgagggcc acaacaccgt gaagctgaag gtgaccaagg gcggccccct
     2041 gcccttcgcc tgggacatcc tgtcccccca gttccagtac ggctccaagg tgtacgtgaa
     2101 gcaccccgcc gacatccccg actacaagaa gctgtccttc cccgagggct tcaagtggga
     2161 gcgcgtgatg aacttcgagg acggcggcgt ggcgaccgtg acccaggact cctccctgca
     2221 ggacggctgc ttcatctaca aggtgaagtt catcggcgtg aacttcccct ccgacggccc
     2281 cgtgatgcag aagaagacca tgggctggga ggcctccacc gagcgcctgt acccccgcga
     2341 cggcgtgctg aagggcgaga cccacaaggc cctgaagctg aaggacggcg gccactacct
     2401 ggtggagttc aagtccatct acatggccaa gaagcccgtg cagctgcccg gctactacta
     2461 cgtggacgcc aagctggaca tcacctccca caacgaggac tacaccatcg tggagcagta
     2521 cgagcgcacc gagggccgcc accacctgtt cctgtagcgg ccgcgactct agatcataat
     2581 cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct
     2641 gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa
     2701 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     2761 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctctagacc
     2821 cagctttctt gtacaaagtg gtgatcgacc ccaactgggg taacctttga gttctctcag
     2881 ttgggggtcg acggggatcc atcacactgg cggccgctcg agcatgcatc tagaattcga
     2941 atggccatgg gacgtcgacc tgaggtaatt ataacccggg ccctatatat ggatccaatt
     3001 gcaatgatca tcatgacaga tctgacaatg ttcagtgcag agactcggct acgcctcgtg
     3061 gactttgaag ttgaccaaca atgtttattc ttacctctaa tagtcctctg tggcaaggtc
     3121 aagattctgt tagaagccaa tgaagaacct ggttgttcaa taacattttg ttcgtctaat
     3181 atttcactac cgcttgacgt tggctgcact tcatgtacct catctataaa cgcttcttct
     3241 gtatcgctct ggacgtcatc ttcacttacg tgatctgata tttcactgtc agaatcctca
     3301 ccaacaagct cgtcatcgct ttgcagaaga gcagagagga tatgctcatc gtctaaagaa
     3361 ctacccattt tattatatat tagtcacgat atctataaca agaaaatata tatataataa
     3421 gttatcacgt aagtagaaca tgaaataaca atataattat cgtatgagtt aaatcttaaa
     3481 agtcacgtaa aagataatca tgcgtcattt tgactcacgc ggtcgttata gttcaaaatc
     3541 agtgacactt accgcattga caagcacgcc tcacgggagc tccaagcggc gactgagatg
     3601 tcctaaatgc acagcgacgg attcgcgcta tttagaaaga gagagcaata tttcaagaat
     3661 gcatgcgtca attttacgca gactatcttt ctagggttaa aaaagatttg cgctttactc
     3721 gacctaaact ttaaacacgt catagaatct tcgtttgaca aaaaccacat tgtggccaag
     3781 ctgtgtgacg cgacgcgcgc taaagaatgg caaaccaagt cgcgcgagcg tcgactctag
     3841 aggatccccg ggtaccgagc tcgaattaat tcgtaatcat gtcatagctg tttcctgtgt
     3901 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     3961 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     4021 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     4081 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     4141 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     4201 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4261 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4321 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4381 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4441 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4501 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4561 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4621 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4681 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     4741 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4801 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4861 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4921 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4981 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     5041 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5101 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5161 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     5221 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5281 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5341 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     5401 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     5461 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     5521 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     5581 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     5641 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     5701 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     5761 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     5821 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     5881 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     5941 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     6001 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     6061 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     6121 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     6181 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     6241 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     6301 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc
     6361 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag
     6421 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt
     6481 gtaaaacgac ggcca
//
